Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
High sensitivity SARS-CoV-2 detection using graphene oxide-multiplex qPCRArticle Published on 2022-11-222022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification applied coronavirus detect detection diagnoses gold standard Graphene Graphene oxide high sensitivity limit of detection molecular multiplex Multiplex qPCR pandemic pathogen detection polymerase chain primer qPCR qPCR assay reduce reference gene RNase P RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 gene SARS-CoV-2. sensitivity single-stranded DNA ssDNA target virus [DOI] 10.1016/j.aca.2022.340533 PMC 바로가기
Multiple genome analytics framework: The case of all SARS-CoV-2 complete variantsArticle Published on 2022-11-202022-11-16 Journal: Journal of biotechnology [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] accelerated Algorithm Algorithms analyses analytics ARPaD bioinformatics Biotechnology can be used center Comparisons Complete computational tool COVID-19 dataset Efficiency framework Genome genome sequence genomic help information introduced knowledge LERP-RSA meta-analyses MPaD MuGA Multiple Multiple genome analytics framework organism palindrome pattern polymerase chain primer problem problems question repeated required resource resources SARS-CoV-2 SARS-CoV-2 genome sequence sequence SPaD. Structure text mining Topic variant variants [DOI] 10.1016/j.jbiotec.2022.09.015 PMC 바로가기
Wastewater surveillance allows early detection of SARS-CoV-2 omicron in North Rhine-Westphalia, GermanyArticle Published on 2022-11-102022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Analysis approach close monitoring collected COVID-19 COVID-19 surveillance demonstrated detection Detection workflow dominant variant Epidemiology evaluate fraction Germany identify Initially K417N Laboratory Local MONITOR mutant Mutation N501Y NGS North omicron Omicron, B.1.1.529 PCR primer public health purified Regulation required resulting RT-qPCR SARS-CoV-2 SARS-CoV-2 epidemiology SARS-CoV-2 monitoring SARS-CoV-2 RNA SARS-CoV-2 variant Sequencing state subsequent Support Surveillance Treatment variant variant of concern was performed wastewater Wastewater-based epidemiology (WBE). [DOI] 10.1016/j.scitotenv.2022.157375 PMC 바로가기
Identification of mutations in SARS-CoV-2 PCR primer regionsArticle Published on 2022-11-042022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 치료기술, 치료제, [키워드] Accuracy Analysis anticipated approach dataset diagnostic Efficiency genomic variation gold standard identification Mutation Neutral omicron overlapping PCR PCR efficiency primer primer set primer sets Region RT-PCR Sample SARS-CoV-2 SARS-CoV-2 PCR SARS-CoV-2 viral strain susceptible target region the SARS-CoV-2 genome variant Variation [DOI] 10.1038/s41598-022-21953-3 PMC 바로가기
Bioinformatics pipeline unveils genetic variability to synthetic vaccine design for Indian SARS-CoV-2 genomesArticle Published on 2022-11-012022-11-15 Journal: International immunopharmacology [Category] SARS, 치료기술, 치료제, [키워드] aligned amino acid change antigenic antigenic epitopes B-cell epitope bioinformatics bioinformatics pipeline can be used clade clades conserved conserved regions criteria epitope Evolution genetic variability genomic HLA allele identify immunogenic MAFFT MHC-I MHC-II Multiple sequence alignment Mutation Nextstrain Non-synonymous Non-Synonymous Signature SNP performed Phylogenetic analysis primer probe Protein Region regions reported resulting SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 pandemic selected SNP SNPs T-cell T-cell epitope T-cell epitopes T-cell epitopes. unique Vaccine Vaccine design virus virus detection virus genome [DOI] 10.1016/j.intimp.2022.109224 PMC 바로가기
SARS-CoV-2 variants of concern and spike protein mutational dynamics in a Swedish cohort during 2021, studied by Nanopore sequencingArticle Published on 2022-10-182022-11-15 Journal: Virology Journal [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] A222V analysed approach ARTIC Network assist bioinformatics can be used circulating variant Cohort COVID-19 COVID-19 pandemic Delta delta variant distribution genomic Health include less mapped Mutation mutations nanopore sequencing National occurred omicron Patient positive primer Protein public health public health measures Q677H S protein SARS-CoV-2 SARS-CoV-2 genomes SARS-CoV-2 variant sequence Sequencing sequencing method spike Spike protein Surveillance Swedish the S protein the SARS-CoV-2 the Spike Uppsala County variant variants variants of concern. variants of interest VoC whole-genome sequencing [DOI] 10.1186/s12985-022-01896-x PMC 바로가기
The Efficient and Practical virus Identification System with ENhanced Sensitivity for Solids (EPISENS-S): A rapid and cost-effective SARS-CoV-2 RNA detection method for routine wastewater surveillance고형물에 대한 감도가 향상된 효율적이고 실용적인 바이러스 식별 시스템(EPISENS-S): 일상적인 폐수 감시를 위한 빠르고 비용 효율적인 SARS-CoV-2 RNA 검출 방법Article Published on 2022-10-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] applicability centrifugation city collected Concentration confirmed COVID-19 case COVID-19 demonstrated Detection sensitivity efficient Epidemiology evaluated examined followed by heat heat-inactivated identification Japan magnitude Mild multiplex normalized pellet Pepper mild mottle virus plant primer Primers qPCR qPCR quantification qPCR. Quantification method quantified ranged reported reverse primer reverse primers RNA concentration RNA concentrations RNA extraction SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection sensitivity Solid subsequent Surveillance System target targets the SARS-CoV-2 Treatment virus wastewater Wastewater-based epidemiology [DOI] 10.1016/j.scitotenv.2022.157101 PMC 바로가기 [Article Type] Article
Accuracy of quality of life questionnaire as a diagnostic tool for SARS-CoV-2 pulmonary sequelaeArticle Published on 2022-10-142022-11-16 Journal: Medicina clínica [Category] COVID19(2023년), SARS, 치료기술, [키워드] age alteration alterations AUC Cardiopulmonary Covid persistente COVID-19 CPET Cuestionario de calidad de vida SF-12 detect diagnostic domain English female first positive follow-up study functional Healthcare system hospital discharge Hospitalization identify Long Long COVID maximum oxygen consumption media Patient PCR performed physical predicted primer Prospective prospectivo Prueba de esfuerzo cardiopulmonar Pruebas funcionales respiratorias pulmonary Pulmonary function Pulmonary function test Quality of life questionnaire reduced SARS-CoV-2 Sensitivity and specificity SF-12 quality of life questionnaire. significantly Spanish survivor Symptom Symptoms utility [DOI] 10.1016/j.medcli.2022.02.007 PMC 바로가기
PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAsArticle Published on 2022-10-142022-11-15 Journal: BMC Bioinformatics [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] assays automated benefit bioinformatics Cas12 nuclease assay Cas12a Combination conserved CRISPR CRISPR-Ca CRISPR-Cas12 Critical demonstrated Dengue diagnostic diagnostics Efficiency facilitated FDA approval field Field forward diagnostics flavivirus genomes genomic HIV In-vitro Intensive isothermal isothermal amplification manual curation Nile nucleases nucleic acid offer parameter Pathogens platform polymorphism PrimedRPA PrimedSherlock primer primer pair Primers qRT-PCR regions RNA robust SARS-CoV-2 Serological assay serotype SHERLOCK significantly specificity strain Strains target targets Vector-borne disease West West Nile wildtype Zika Zika. [DOI] 10.1186/s12859-022-04968-5 PMC 바로가기